Patterns of three-dimensional growth in vitro in collagen-coated cellulose sponge: carcinomas and embryonic tissues.

Several t ransplantable tumors and chick embryonic tissues were cultured in collagen-coated cellulose sponge. The growth obtained in this matr ix was compared with that obtained in cellulose sponge alone and in collagen sponge alone. The tumors included a mouse mammary adenoeareinoma (C3HBA), a squaincus cell carcinoma of the mouse cervix (D-98), a mouse ascites hepatoma (Sato A H #134) , a rat ascites hepatoma (Yoshida #7974) , and a human adenocareinoma of the colon (tt .Ad.-1). The embryonic tissues were intestine, hmg, and heart. All the sponges were examined in histologic section. The best pat terns of migratory outgrowth were seen in collagencoated cellulose sponge. Conventional histologic criteria were used to identify the cellular components of the newly formed tissues.